Figure 7.

TRIM25 regulates the stability of IAV mRNAs. HEK293 WT, HEK293 TRIM25 KO, HEK293 TRIM25 KO + TRIM25 WT, HEK293 TRIM25 KO + TRIM25ΔRBD and HEK293 TRIM25 KO + TRIM25ΔRING were infected with IAV PR8 R38K41A and were treated with the viral polymerase inhibitor Favipiravir (T-705). The changes in the amount of viral mRNA segments were measured using qRT-PCR in the cells at 48 h after Favipiravir treatment. To quantify positive sense viral mRNAs a qRT-PCR with an oligo dT primer was used for the RT. Primers for specific viral segments were used for amplification of all 8 segments. The means and SDs of five biological replicates are shown. Statistical significance was calculated using ANOVA on ranks.