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Complementation of phenotype. (A) Agar plate images of the complementation of the phenotype in Δmpr by GFP (Δmpr::GFP) and mpr (Δmpr::mpr) are shown. (B) Growth curve of M. smegmatis wildtype, mpr knockout, and knockout complemented with mpr under phage infection condition is shown. Cultures were allowed to grow till OD600 ∼0.8 (normalized) and infected (‘*’) with high titre phage. OD600 was monitored at every 6 h and plotted.
