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. 2022 Jun 17;50(12):6938–6952. doi: 10.1093/nar/gkac505

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Nuclease activity of Mpr and its truncations. Agarose gel image showing nuclease activity of full length Mpr and its truncations on blunt end linear dsDNA is presented. Δ32Mpr, Δ50Mpr and ΔTMMpr are not able to digest DNA, whereas Mpr, Δ26Mpr, ΔCMpr and TMGFP can digest linear DNA. ΔTMMpr is able to bind to dsDNA, but is unable to digest it, resulting in mobility shift on agarose gel. Dialysis buffer is used as a control; GFP is used as a nonspecific control protein.