Table 1.
List of constructs used in the study
| S. No. | Name of plasmid | Backbone | Promoter | Type | Reporter tag | Source |
|---|---|---|---|---|---|---|
| 1. | pET21b -mpr | pET21b | T7 promoter | replicon | 6xHis | This study |
| 2. | pET21b-Δ26mpr | pET21b | T7 promoter | replicon | 6xHis | This study |
| 3. | pET21b-Δ32mpr | pET21b | T7 promoter | replicon | 6xHis | This study |
| 4. | pET21b-Δ50mpr | pET21b | T7 promoter | replicon | 6xHis | This study |
| 5. | pMSQSCHS-ΔTMmpr | pMSQSCHS | T7 promoter | replicon | 6xHis | This study |
| 6. | pMSQSNHS-TMGFP | pMSQSNHS | T7 promoter | replicon | 6xHis | This study |
| 7. | pMSQSCHS-ΔCmpr | pMSQSCHS | T7 promoter | replicon | 6xHis | This study |
| 8. | pMSQSCHS-GFP | pMSQSCHS | T7 promoter | replicon | 6xHis | This study |
| 9. | pET21b-M1 | pET21b-mpr | T7 promoter | replicon | 6xHis | This study |
| 10. | pET21b-M2 | pET21b-mpr | T7 promoter | replicon | 6xHis | This study |
| 11. | pET21b-M3 | pET21b-mpr | T7 promoter | replicon | 6xHis | This study |
| 12. | pET21b-M6 | pET21b-mpr | T7 promoter | replicon | 6xHis | This study |
| 13. | pET21b-M8 | pET21b-mpr | T7 promoter | replicon | 6xHis | This study |
| 14. | pET21b-M9 | pET21b-mpr | T7 promoter | replicon | 6xHis | This study |
| 15. | pET21b-M10 | pET21b-mpr | T7 promoter | replicon | 6xHis | This Study |
| 16. | pMA-mprHis | pMA-His | acetamidase | replicon | 6xHis | This study |
| 17. | pMA-mprGFP | pMA-GFP | acetamidase | replicon | GFP | This study |
| 19. | pMA-Δ50GFP | pMA-GFP | acetamidase | replicon | GFP | This study |
| 20. | pMA-ΔTMGFP | pMA-GFP | acetamidase | replicon | GFP | This study |
| 21. | pMA-His | pMA-His | acetamidase | replicon | 6xHis | Lab material |
| 22. | pMIA-GST | pMA-GST | acetamidase | Integration | GST | This study |
| 23. | pMIA-mpr-His | pMA-His | acetamidase | Integration | 6xHis | This study |
| 24. | pMIA-ΔCmpr | pMA-GFP | acetamidase | Integration | GFP | This study |
| 25. | pMIM-mpr | pMIA-His | mpr | Integration | 6xHis | This study |
| 26. | pMIM-GFP | pMIA-GFP | mpr | Integration | GFP | This study |
| 27. | pMIM-Δ26mpr | pMIM-mpr | mpr | Integration | 6xHis | This study |
| 28. | pMIM-ΔCmpr | pMIM-mpr | mpr | Integration | GFP | This study |
| 29. | pMM-GFP | pMA-GFP | mpr | replicon | GFP | This study |
| 30. | pMM-mpr | pMH-His | mpr | replicon | 6xHis | This study |
| 31. | pMH-GFP | pMH-GFP | hsp60 | replicon | GFP | Lab material |
| 32. | pSD5b | pSD5b | *NP | replicon | lacZ | Lab material |
| 33. | pSD5bPrmpr | pSD5b | mpr | replicon | lacZ | Lab material |
*NP, no promoter present in the plasmid.