FIG. 4.

Cultivation of B. licheniformis ATCC 9945 (A) and B. subtilis 1551 (B) under fed batch conditions. The strains were cultivated at 35°C for 144 h in 500-ml conical flasks equipped with three baffles containing 100 ml of medium composed of 54% (vol/vol) tap water, 33% (vol/vol) medium E, and 13% (vol/vol) particle-free liquid manure. At the times indicated by arrows, 0.13 volume of particle-free liquid manure was added. Symbols: ●, optical density at 600 nm; ▵, ammonium concentration; □, PGA concentration.