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. Author manuscript; available in PMC: 2022 Sep 25.
Published in final edited form as: Mucosal Immunol. 2022 Mar 25;15(4):683–697. doi: 10.1038/s41385-022-00502-1

Fig 6. High VDR levels increased Claudin-5 at the protein and mRNA level in vitro.

Fig 6.

(A) Claudin-5 mRNA and (B) protein levels were increased after 24-hour vitamin D3 treatment at 20 nM in SKCO15 cells (data are expressed as mean ± SD. Student’s t-test, n = 3). (C) Claudin-5 mRNA and (D) protein levels were higher in vitamin D3- treated VDR+/+ mice. VDR+/+ mice (6-8 weeks) were gavaged by 0.2 μg vitamin D3 in 0.1 ml corn oil 3 times per week for 4 weeks (data are expressed as mean ± SD. Student’s t-test, n= 5 mice/group). (E) The micrographs showed representative human colonoids treated with Vit D3 (20 nM) for the indicated time points. (F) Claudin-5 mRNA and (G) protein levels were increased after vitamin D3 treatment in human colonoids (data are expressed as mean ± SD, n= 5, one-way ANOVA test). All p values are shown in the figure.