Figure 1. CXCR5⁺ CD4⁺ or CD3⁺ T_fh cells in the lymph node of a B6 mouse five days after the immunization with NP₃₃-CGG with alum in the hind footpads express act. caspase-1⁺ and IL-1β.

(A) Many of the act. caspase-1⁺ Tfh cells in the interface between T cell B cell zones and in the dark zone of the GC are identified by arrows. The inset shows interaction between act. caspase-1⁺ Tfh cells with IgD⁺ B cells. (B) CD4⁺CXCR5⁺ T cells as seen by the ImageStreamX MKII imaging flow cytometer with act. caspase-1 stained green, IL-1β red, CD4 purple and CXCR5 blue. (C) A CD3⁺CXCR5⁺ T cell stained for act. caspase-1 and IL-1β. (D) CD3⁺CXCR5⁺Tfh and caspase-1⁺ IL1β⁺ Tfh were counted in each 300 μm × 300 μm area.