Skip to main content
. Author manuscript; available in PMC: 2022 Jul 8.
Published in final edited form as: Ann Rheum Dis. 2022 Apr 12;81(7):1006–1012. doi: 10.1136/annrheumdis-2021-221985

Figure 4.

Figure 4.

(A) B6.NLRP3fl/flCreLck mice showed reduced Ab response to soluble Ag. Groups of 6 WT (B6) and 10 mutant mice were immunized subcutaneously in the neck and in the footpad with NP33-CGG (100μg/mouse) without alum. Mice were bled on days 7 and 14 after immunization. Sera were diluted at 1:1000. Anti-NP Ab were quantified with ELISA using plate-bound NP14-BSA and NP2-BSA for low and high affinity Ab respectively. Results are expressed as mean ±SD. *p<0.05, **p<0.01 and ***p<0.001. (B) Lack of proliferating cells in the splenic germinal centers. (a-d) Two splenic germinal centers with Ki67+ cells in B6.NLRP3fl/fl (a&b 10X and c&d 20X) five days after immunization with NP33-CGG. (e-h) Absence of Ki67+ cells in the spleen of a B6.NLRP3fl/flCreLck mouse in that NLRP3 is specifically deleted from T cells.