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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Am J Transplant. 2022 Apr 15;22(7):1766–1778. doi: 10.1111/ajt.17038

Figure 2.

Figure 2.

ATG treatment induces antigen-specific Tfh cells expansion. (A) C57Bl/6 mice were subcutaneously immunized with NP-OVA + CFA and intraperitoneally treated with 500 μg of murine ATG or IgG control on days 0 and 4. On day 8 after immunization, lymph nodes were analyzed by flow cytometry. (B) Representative flow cytometry contour plots of CD4+ T cells in lymph nodes gated in Dump- live cells. (C) The frequency and absolute cell numbers per lymph node of total CD4+ T cells. (D) Representative flow cytometry contour plots of Tfh (CD4+CXCR5+PD-1+) cells in lymph nodes. (E) The frequency and absolute cell number per lymph node of Tfh (CD4+CXCR5+PD-1+) cells. (F) Representative contour plots of and frequency of OVA323–339-tetramer+ cells in Tfh (CD4+CXCR5+PD-1+) and non-Tfh (CD4+CXCR5- PD-1-) cells. (A-F) Red dots represent the ATG-treated mice, and the black dots represent the IgG-treated mice. Data as mean ± SD are shown (pooled data from three independent experiments, with n = 5 per group; t-test). (C and E) Statistic by t-test. (F) Statistic by Two-way ANOVA with Tukey multiple comparisons test.