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. Author manuscript; available in PMC: 2023 Jul 5.
Published in final edited form as: Cell Metab. 2022 Jun 15;34(7):1064–1078.e6. doi: 10.1016/j.cmet.2022.05.010

Figure 7. Effects of SGLT2 inhibitors on the S1 vs. the S2 segment.

Figure 7.

(A) Reclustering of the PCT into S1 and S2 segments.

(B) Density plot showing that Slc5a2 expression is limited to the S1 segment.

(C) Upset plot showing the diabetes-specific DEGs that are rescued by each treatment, divided according to whether they are unique to each treatment or shared across treatments.

(D) Expression of Srsf7 in S1 and S2 across the day2 groups.

(E and F) Knockdown efficiency of siRNA as corroborated by qPCR and western blot.

(G) Volcano plot showing the differential genes in the comparison of Srsf7 siRNA and scramble.

(H) Enriched pathways from gene ontology analysis. NES: normalized enrichment score.

(I and J) Expression of the selected genes in RPTECs and mouse model. All selected genes are DEGs identified by comparing Srsf7 siRNA versus scramble in RPTECs, AAV versus db/m and drug treatments versus AAV in mouse PT-S1.