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. 2022 May 16;21(6):e12816. doi: 10.1111/gbb.12816

FIGURE 1.

FIGURE 1

Validation of global NSG1 knockout (KO) in null animals. (A) Agarose gel showing PCR‐based genotype analysis of a NSG1 heterozygote (lane 2), four NSG1 wild‐type (lanes 3–6), and four NSG1 KO animals (lanes 7–10). DNA ladder (lane 1) is show for molecular weight reference. (B) Western blot illustrating labeling of NSG1 (green) and NSG2 (red) from four wild‐type (left‐most lanes) and four KO animals (right‐most lanes) used in behavioral testing. (C‐D) Immunohistochemical staining of NSG1 (green), NSG2 (red), and DAPI (blue) on parasagittal brain sections from a wild‐type (C) and KO animal (D). Note the robust cerebellar staining of NSG1 antibody in wild‐type brain section while NSG2 signal is relatively low, as well as lack of NSG1 signal in a section from an NSG1 KO animal (bottom right panel in D)