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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Mol Carcinog. 2022 May 5;61(8):752–763. doi: 10.1002/mc.23415

Figure 6.

Figure 6.

The effect of stable knockdown of MCT1 protein on DATS-mediated inhibition of bCSC. (A) Representative flow histograms for the CD49fhigh/CD44high population in SUM159 cells stably transfected with a shCont or a shMCT1 after treatment with DMSO or DATS (2.5 μM) for 72 hours. (B) Bar graph shows the percentage of the CD49fhigh/CD44high population when compared to respective control. (mean ± S.D., n = 3) (C) Representative flow histograms for ALDH1 activity in SUM159 cells stably transfected with a shCont or a shMCT1 after 72-h treatment with DMSO or DATS (2.5 μM). (D) The graph shows the quantitation of ALDH1 activity when compared to respective control (mean ± S.D., n = 3). (E) Representative mammosphere images after 7 days of treatment with DMSO or DATS (5 μΜ) in SUM159 cells stably transfected with a shCont or a shMCT1 (100x magnification). (F) Quantitation of mammosphere formation when compared to respective to control (mean ± S.D., n = 3). * P < 0.05 between the indicated groups by one-way ANOVA followed by Bonferroni’s multiple comparisons test. Similar results were observed in independent experiments.