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. 2022 Jul 7;13:3913. doi: 10.1038/s41467-022-31635-3

Fig. 8. Brain aromatase regulates hippocampal memory.

Fig. 8

A The cognitive effect of aromatase blockade and βE2 recovery was studied using the novel object location test (NOL) with 10 min inter-trial interval between the familiarization and test sessions. ∫ B Fourteen days after ovariectomy (OVX), adult female mice received daily intraperitoneal (i.p.) injections of vehicle (Control), the aromatase blocker letrozole (LTZ, Arom Block) or the aromatase blocker LTZ+ βE2 (Recovery) for 5 days. Mice performed the NOL test 90 min after the last injection. Graph represents population data. Discrimination index > 0 indicates preferential exploration of the displaced object during the test session. Dotted line represents chance levels. One sample t test (indicated below the graph): Control, t(11) = 3.41, #p = 0.006, Arom Block, t (18) = 0.14, n.s. p = 0.89; Recovery, t (9) = 3.67, #p = 0.005. One-way ANOVA (indicated above the graph): F(2, 38) = 9.12, p = 0.0006; Bonferroni’s comparison tests, C vs. Arom Block p = 0.03, Arom Block vs. Recovery p = 0.0003; n = 12, 19, 10 mice. C Adult male mice received daily intraperitoneal injections of vehicle (Control) or the aromatase blocker letrozole (Arom Block) for 5 days. Mice performed the NOL test 90 min after the last injection. Graph represents population data. Discrimination index > 0 indicates preferential exploration of the displaced object during the test session. Dotted line represents chance levels. One sample t test (indicated below the graph): Control, t (9) = 3.82, #p = 0.004; Arom Block, t(12) = 2.89, #p = 0.013. Unpaired two-tailed t test (indicated above the graph), t(21) = 0.96, n.s. p = 0.35; n = 10, 13 mice per group. Graphs represent mean ± SEM (line and bars) and individual values (circles) for each experimental condition. * and #p < 0.05; n.s. p > 0.05. Source data are provided as a Source Data file.