Fig. 3.

MiR-187-3p directly targets LRFN1-3′-UTR and modulates the prognostic LRFN1 expression. A A putative binding site of miR-187-3p in the 3′-UTR of LRFN1. B Luciferase assay was performed to investigate whether miR-187-3p binds to a putative binding site in the 3′-UTR of LRFN1. C RT-qPCR and Western Blot were performed to assess the transcriptional and protein expression level alteration of LRFN1. D Prognostic implication of LRFN1 in 30 different tumors from TCGA database using log rank test [− log10(P-value)]. E Kaplan–Meier survival plots were used to evaluate predictive value of LRFN1 expression in OS and PFS for 504 patients with ccRCC from TCGA cohort. F Kaplan–Meier survival plots suggested prognostic role of LRFN1 expression for ccRCC patients from CPTAC cohort. G Kaplan–Meier survival plots suggested prognostic role of LRFN1 expression for ccRCC patients from RECA-EU cohorts. *P < 0.05; ***P < 0.001