Fig. 4.

LRFN1 rescues ccRCC cells proliferation, migration after miR-187-3p mimic transfection. A RT-qPCR was performed to assess LRFN1 mRNA expression in A498 and 786O cells. B, C RT-qPCR and Western Blot was performed to assess LRFN1 mRNA and protein expression in A498 and 786O cells. D, E CCK-8 assay was performed to measure cell proliferation in A498 and 786O cells. F, G Transwell assay was performed to determine the migration ability of A498 and 786O cells overexpressing LRFN1 and mimics, mimics + LRFN1 groups. Statistical significance was calculated using Student’s t test *P < 0.05; **P < 0.01; ***P < 0.001