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. 2022 Jul 7;13(7):586. doi: 10.1038/s41419-022-05035-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A from left to right, CHST15 blotting bands represent sporadic MPN patients; healthy controls and familial MPN, respectively. B CHST15 protein expression on HEL cells with wild-type CHST15 (WT) and mutant CHST15 gene(Mut) and non - transfected HEL cells (HEL). C Quantitative RT-PCR analysis of CHST15 mRNA. Left panel: from left to right, F1-1, F1-2, F2-1, F2-2 indicate familial PMF; S1, S2, S3 indicate sporadic PMF; N1, N2, N3 indicate normal individuals. Results are means of three independent experiments. Right panel: when the mRNA expression levels were compared by pairwise comparison, the results showed that there was significantly depressed expression on CHST15 mRNA in familial MPN (p < 0.05).