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. 2022 Jul 7;13(7):586. doi: 10.1038/s41419-022-05035-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Coimmunoprecipitation between JAK2 and CHST15 on HELcells harbouring CHET15 mutation (CHST15^R456fs) or wild-type CHST15. left panel:anti-JAK2 antibody; right panel:anti- Flag-CHST15 antibody. Input, non-immunoprecipitated cell lysates; IgG, control IP with isotype antibody. B Coimmunoprecipitation between JAK2 and CHST15 on primary PBMNCs of patients with familial MPN (left panel: 1, 2, Familial 1. PMF: 3, 4. Familial 3. ET; 5,6. Familial 2, PMF.) or sporadic MPN (right panel: 1’, 2’, 3’: sporadic MPN with JAK2V617F; 4’, 5’, 6’: MPN with CALR mutation; 7’,8’,9’: MPN without mutation- triple negative). C Immunofluorescent colocalization of FLAG-tagged-CHST15 and JAK2. 4′,6-diamidino-2-phenylindole (DAPI) wasused for nuclear staining. The results show that both JAK2 and CHST15 are mainly found on cytomembrane and cytoplasm, and form co-precipitatation with granular shape between CHET15 and JAK2, suggesting exists in physical interaction both JAK2 and CHST15 (wild type) especially CHST15^R456fs mutant.