FIGURE 4.

(Left) Illustration of AIEgen molecules in solution and aggregated state, and their corresponding simplified Jablonski diagrams showing the effect of motion restriction on the radiative (k_r) and non-radiative (k_nr) relaxation pathways after 1PE or 2PE. (Right) Example of in vivo application of 2P microscopy with NIR-II-responsive AIEDots used to reconstruct images of a mouse brain. The 3D reconstruction of mouse brain vasculature is reprinted with permission from ref. (Qi et al., 2018). Copyright 2018 American Chemical Society.