Figure 4.

H3H-Luz tested with metabolic burden-reduced TXTL. (A) The schematic of how H3H-carrying TXTL works. The plasmid coding H3H gene under the sigma 70 promoter is transformed into E. coli Rosseta 2 strain. The cell-free extract is made with that strain; thus, the extract contains H3H. Once Luz is expressed in the TXTL, Luz produces luminescence by coordinating with H3H. (B) Luminescence measurement in the H3H pre-containing TXTL. Luz plasmids were incubated with hispidin at 30 °C in TXTL. The Luz plasmid containing reaction (yellow dots) generated light during the TXTL reaction, while the reaction without Luz plasmid did not (black dots.) The graphs show means with error bars that signify SEM (n = 3).