Figure 5.

Luz’s capacity as reporter gene fusions. (A) Luminescence measurement of the H3H-Luz system with the eGFP fused Luz constructs. H3H and Luz were expressed in TXTL. After the expression, hispidin and NADPH were added, followed by luminescence measurement. (B) Fluorescence measurement of the eGFP fused Luz. Luz proteins were expressed in TXTL and the fluorescence was measured. Luz, Luz luciferase without a fusion protein; N-GFP, N-terminal eGFP fusion with Luz; C-GFP, C-terminal eGFP fusion with Luz; Control, reaction without enzyme expression. The graphs show means with error bars that signify SEM (n = 3).