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The validation data for the determination of phyllanthin and hypophyllanthin by elaborated HPLC–UV method.
| Validation parameters | Phyllanthin | Hypophyllanthin |
|---|---|---|
| Linearity (linear regression equation, regression coefficient) | y = 0.000123995x + 0.295; r = 0.9998 | y = 0.00016344x + 1.727; r = 0.9996 |
| Limit of detection(LOD) [ng/ml] | 105.9 | 138.6 |
| limit of quantification (LOQ) [ng/ml] | 353 | 462 |
| Intra-day precission [RSD, %] | 0.38 | 0.17 |
| Inter-day [RSD, %] | 0.46 | 0.48 |
| repeatability [RSD, %] | 2.98 | 2.95 |
