Table 5.
Effect of 2iP supplementation in combination with BAP, Kin or TDZ on the development of P. amarus shoot culture.
| Plant growth regulators (mg/L) | Proliferation rate (mean ± SD) | Shoot lenght (cm) (mean ± SD) | Root length (cm) (mean ± SD) | Shoot number/explant (mean ± SD) | % rooting plantlets | Callus [mm] (mean ± SD) |
|---|---|---|---|---|---|---|
| Control (MS0) | 1.0 ± 0.00a | 7.97 ± 1.08a | 3.84 ± 0.90a | 9.44 ± 2.57a | 100 | – |
|
BAP 1.0 2iP 0.25 |
1.43 ± 1.10b | 3.46 ± 1.34c | 0.58 ± 0.73d | 1.84 ± 2.74e | 47.05 | 10.73 ± 3.48d |
|
BAP 1.0 2iP 0.5 |
1.42 ± 1.09b | 3.93 ± 1.41c | 0.80 ± 0.85c | 2.68 ± 3.32d | 58.00 | 10.46 ± 3.77d |
|
BAP 1.0 2iP 1.0 |
1.22 ± 0.87a | 4.64 ± 2.07bc | 0.85 ± 0.96c | 3.02 ± 4.15d | 57.14 | 10.35 ± 3.82d |
|
BAP 1.0 2iP 2.0 |
1.65 ± 1.26b | 4.02 ± 1.49c | 0.46 ± 0.57d | 1.98 ± 2.77e | 47.06 | 9.22 ± 4.36d |
|
Kin 1.0 2iP 0.25 |
1.08 ± 0.34a | 5.14 ± 1.52b | 1.71 ± 1.21b | 6.57 ± 5.14b | 88.24 | 5.90 ± 2.33c |
|
Kin 1.0 2iP 0.5 |
1.27 ± 0.72a | 4.70 ± 1.29bc | 1.21 ± 0.95c | 5.25 ± 4.55c | 86.54 | 5.38 ± 2.28c |
|
Kin 1.0 2iP 1.0 |
1.24 ± 0.74a | 4.24 ± 1.06c | 0.71 ± 0.57c | 3.06 ± 3.46d | 76.00 | 5.22 ± 2.09c |
|
Kin 1.0 2iP 2.0 |
1.43 ± 0.92b | 4.31 ± 1.37c | 0.38 ± 0.51d | 1.76 ± 2.83e | 45.10 | 5.90 ± 2.52c |
|
TDZ 0.05 2iP 0.25 |
2.31 ± 1.21cd | 0.32 ± 0.09e | – | – | – | 12.06 ± 4.47e |
|
TDZ 0.05 2iP 0.5 |
2.43 ± 1.29cd | 0.47 ± 0.36e | – | – | – | 11.98 ± 6.07d |
|
TDZ 0.05 2iP 1.0 |
2.47 ± 1.57cd | 0.51 ± 0.39e | – | – | – | 13.59 ± 6.03de |
|
TDZ 0.05 2iP 2.0 |
2.84 ± 1.68d | 0.51 ± 0.24e | – | – | – | 13.65 ± 5.36e |
The results are the arithmetic means of ≥ 50 trials ± SD The values in each column marked with different letters (a, b, c …) indicate statistically significant differences (p < 0.05; Tukey's RIR test).