Table 6.
Effect of 2iP and IBA supplementation on the development of P. amarus shoot culture.
| Plant growth regulators (mg/L) | Proliferation rate (mean ± SD) | Shoot lenght (cm) (mean ± SD) | Root length (cm) (mean ± SD) | Shoot number/explant (mean ± SD) | % rooting plantlets | Callus [mm] (mean ± SD) |
|---|---|---|---|---|---|---|
| Control (MS0) | 1.0 ± 0.00a | 7.97 ± 1.08a | 3.84 ± 0.90a | 9.44 ± 2.57a | 100 | – |
| IBA 0.25 | 1.0 ± 0.00a | 6.86 ± 2.51ab | 2.86 ± 1.61a | 10.16 ± 6.15a | 98 | – |
| IBA 0.5 | 1.0 ± 0.00a | 7.44 ± 2.99a | 2.30 ± 1.20b | 7.82 ± 4.88b | 98 | – |
| IBA 1.0 | 1.0 ± 0.00a | 6.34 ± 2.48b | 1.61 ± 0.71b | 7.59 ± 5.31b | 100 | – |
| IBA 2.0 | 1.0 ± 0.00a | 4.89 ± 2.14b | 1.04 ± 0.28c | 7.03 ± 3.92e | 97 | – |
|
2iP 0.25 IBA 0.5 |
1.07 ± 0.25a | 6.31 ± 2.87b | 2.02 ± 0.69b | 13.15 ± 7.93g | 100 | 5.96 ± 1.40c |
|
2iP 0.5 IBA 0.5 |
1.16 ± 0.47a | 5.87 ± 2.08b | 1.86 ± 0.85b | 12.22 ± 5.47g | 100 | 6.30 ± 2.07c |
|
2iP 1.0 IBA 0.5 |
1.24 ± 0.57a | 5.27 ± 2.90b | 1.83 ± 0.91b | 10.71 ± 5.21a | 100 | 7.73 ± 2.71b |
|
2iP 2.0 IBA 0.5 |
1.42 ± 0.60b | 4.90 ± 3.02b | 1.83 ± 1.06b | 9.75 ± 4.72a | 100 | 7.97 ± 2.02b |
The results are the arithmetic means of ≥ 50 trials ± SD The values in each column marked with different letters (a, b, c …) indicate statistically significant differences (p < 0.05; Tukey's RIR test).