TABLE 3.
Production of the hydroxamate siderophores and susceptibility to ALS84 and agrocin 84 under conditions of iron sufficiency and iron deficiency
| Growth mediuma | Producer strain | Siderophore productionb | Antibiosis assayc
|
Antibiotic activity produced | |||
|---|---|---|---|---|---|---|---|
| Indicator strain | Assay medium
|
||||||
| ST | MG | MGF | |||||
| ST | K84 | − | C58 | + | + | + | Agrocin 84 |
| NT1 | − | − | − | ||||
| K84 Agr− | − | C58 | − | − | − | None | |
| NT1 | − | − | − | ||||
| MG or CM9 | K84 | + | C58 | + | + | + | Agrocin 84 + ALS84 |
| NT1 | − | + | − | ||||
| K84 Agr− | + | C58 | − | + | − | ALS84 | |
| NT1 | − | + | − | ||||
a
ST medium contains nonlimiting amounts of iron; MG and CM9 media contain limiting amounts of iron.
b
Production of the hydroxamate siderophore was assessed by the CAS and Csàky tests as described in Materials and Methods. +, production; −, no detectable production.
c
Tested by the plate overlay assay as described in Materials and Methods. +, zone of growth inhibition produced; −, no detectable zone of growth inhibition produced.