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From (A–J), hybrid mRNA (TP53)—miRNA upregulated. The upper and lower sequences represent the miRNA in the 3′-5′ sense and 5′-3′ sense hybridization sites in the mRNA, repectively. The numbers at the ends of the mRNA show the starting and ending position of the hybridization sites. Dots between the sequences indicate the paired nucleotides. Nucleotides in red mark the “seed” sequence in miRNA. The energy resulting from the hybridization was calculated by the RNAhybrid algorithm.
