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. 2022 Mar 21;30(7):2618–2632. doi: 10.1016/j.ymthe.2022.03.011

Figure 1.

Figure 1

Mice surviving CLP exhibit anxiety-like behavior, memory impairment, and a decrease of dendritic spine density

(A and B) Sham (n = 23) and CLP (n = 27) mice were subjected to open-field test. (A) Representative tracks and heatmap are shown. (B) The frequency, time in the center zone, and the first time for the mice to go to the center zone were analyzed (∗p < 0.05). (C) Sham (n = 12) and CLP (n = 16) mice were subjected to a contextual fear-conditioning test, and freezing behavior was analyzed ( ∗p < 0.05). (D) Differential expression of IEGs in neuronal cells between Sham and CLP mice were analyzed at 24 h after surgery (∗p < 0.05, n = 3 mice/group). (E) The PSD-95 protein levels were determined in the hippocampus of mice at 24 h after Sham or CLP operation (∗p < 0.05, n = 3 mice/group). (F) Neuron dendrite from Sham and CLP mice were stained with Golgi stain (left). Box plots of dendritic spine number in Sham and CLP groups were analyzed at 8 weeks post-surgery (right). Scale bar, 20 μm. ∗p < 0.05, n = 3–4 mice/group.