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. 2022 Mar 21;30(7):2618–2632. doi: 10.1016/j.ymthe.2022.03.011

Figure 5.

Figure 5

Neat1/Hbb axis suppresses PSD-95 expression and dendritic spine density

(A) The Neat1 levels were measured in N2a cells after transfection with Neat1 GapmeR for 48 h ( ∗p < 0.05, n = 4). (B) The PSD-95 protein levels were determined after transfection of N2a cells with Neat1 GapmeR for 48h ( ∗p < 0.05, n = 6). (C) The protein levels of PSD-95 were detected after transfection of the primary neuronal cells with Neat1 GapmeR for 24 h (∗p < 0.05, n = 4). (D) Primary neurons were transfected with control or Neat1 GapmeR for 24 h. The dendritic spine numbers were analyzed by immunostaining to label PSD-95 puncta and axons ( ∗∗p < 0.01, n = 6; PSD-95, green; βⅢ-tubulin, red. Scale bar, 5 μm. (E and F) The protein levels of Hbb and PSD-95 were determined after transfection of the N2a cells (E) and primary neuron cells (F) with control or Hbb GapmeR ( ∗p < 0.05, ∗ ∗p < 0.01, n = 5–6). (G) Control or Hbb GapmeR was transfected into primary neuronal cells for 24 h. The dendritic spine numbers were analyzed by immunostaining to label PSD-95 puncta and axons (∗ ∗p < 0.01, n = 6; PSD-95, green; βⅢ-tubulin, red. Scale bar, 5 μm.