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. 2022 Mar 28;30(7):2554–2567. doi: 10.1016/j.ymthe.2022.03.015

Figure 2.

Figure 2

SCD1 is required for matrix stiffness-induced invasion in HCC cells

HCC cells expressing control (shControl) or SCD1 shRNA (shSCD1) were cultured on polyacrylamide hydrogels with the indicated rigidities (1.6 and 25.6 kPa) and plastic plates in 3D Matrigel overlay culture. (A) Efficiency of SCD1 knockdown was probed by western blot. (B) Bright-field images of HCC cells. The in vitro invasion of HCC cells is quantified by the degree of cell scattering using ImageJ software. (C) Expression of EMT-related markers E-cadherin and N-cadherin was assessed by western blot. HCC cells were treated with or without oleic acid (OA) (the product of SCD1). (D) Ratio of monounsaturated/saturated fatty acids in all lipid species. (E) Phospholipid levels were determined by lipidomic mass spectrometry. (F) Hue-saturation-brightness images of HCC cells. The value of the minimum color (blue) was set to generalized polarization (GP) = −1, and the maximum (red) was set to 1. ∗p < 0.05, ∗∗∗p < 0.001.