Figure 7.

Knockdown of SCD1 suppresses HCC invasion and metastasis in vivo

(A) The growth curve of subcutaneous tumors derived from SCD1 knockdown or control MHCC97H cells (shSCD1 versus shControl) in nude mice. (B) Weight of subcutaneous tumors from SCD1 knockdown or control MHCC97H cells (shSCD1 versus shControl). (C) Expression of SCD1, α-SMA, Ki67, and EMT-related markers (E-cadherin and N-cadherin) was analyzed by immunohistochemistry. (D) Subcutaneous tumors were orthotopically implanted into the livers of nude mice and allowed tumor development. (E) In situ liver tumors derived from SCD1 knockdown versus shControl MHCC97H cells presented a less invasive tumor edge and a decrease in the number of distant lung metastases, as indicated by H&E staining. ∗∗p < 0.01, ∗∗∗p < 0.001.