Figure 2.
FOSL1 promotes transformation of PN GSCs into an MES state
(A) Representative FACS plots of CD44+ subpopulation in PN 35 and PN 182 GSCs transduced with vector control or FOSL1. Median fluorescence intensity of CD44 is shown on the right. (B) IB analysis of FOSL1, CD44, C/EBPβ, TAZ, p-STAT3 (Tyr705), and STAT3 in PN 35 and PN 182 GSCs ectopically expressing FOSL1 or vector control. α-Tubulin was used as internal control. (C) Limiting dilution neurosphere-forming assay in PN 35 and PN 182 GSCs transduced with FOSL1 or vector control. (D) Representative BLI images of mice bearing xenografts derived from luciferase-expressing PN 35 and PN 182 GSCs transduced with FOSL1 or vector control. Colored scale bars represent photons/s/cm2/steradian. (E) Representative H&E-stained brain sections and IHC-staining images of FOSL1, CD44, and YKL40 in mice bearing xenografts derived from PN 35 and PN 182 GSCs with indicated modifications. Red arrows indicate tumors. Scale bars, 1 mm (H&E staining) and 25 μm (IHC staining). (F) Kaplan-Meier survival curves of mice intracranially implanted with PN 35 and PN 182 GSCs with indicated modifications (n = 8).