TABLE 1.

Characteristics and reported applications of different microfluidic devices used in neuroscience research (Kamudzandu et al., 2019). Adapted from Kamudzandu et al. (2019) (copyright Biomedical Physics & Engineering Express) (Peyrin et al., 2011); Extracted and adapted and from Peyrin et al. (2011). (copyright Lab on a Chip) (Lassus et al., 2018); Extracted and adapted from Lassus et al. (2018) (copyright Scientific Reports). Schematic visualization: 1 and 3: inlets; 2 and 4: outlets; 5 and 6: channels; 7: microchannels/microgrooves.

Schematic visualization	1 node	2 nodes	3 nodes	4+ nodes
References architectures	Maisonneuve et al. (2021a)	Maisonneuve et al. (2021a)	Maisonneuve et al. (2021a)	Maisonneuve et al. (2021b)
Fabrication methods	3D printing (Amin et al., 2016)	Photolithography (Taylor et al., 2005)	Photolithography (Moutaux et al., 2018; Virlogeux et al., 2018)	3D printing (Amin et al., 2016)
Materials	PDMS	PDMS, COC	PMDS, COC	PDMS
Add-on directionality	N/A	(Peyrin et al., 2011)	(Lassus et al., 2018)	(Kamudzandu et al., 2019)
Applications examples	Functional recording	Co-culture, (ex: Neuron-neuron, NMJ neuro-glial)	Axotomy, synaptic injury	Prion like propagation diseases, functional recordings
Electrophysiology	Fluorescent imaging, MEA, HD-MEA	Fluorescent imaging, MEA	MEA	MEA