Figure 1.

EGFR, EGFRvIII, and IL13Rα2 co-express in GBM

(A) Flow cytometry analyses of EGFRvIII and EGFR expression in resected glioma tissues (left). Statistically significant differences were determined using two-tailed paired t test. Flow panels of red dots indicated EGFR expression along the x axis and EGFRvIII expression along the y axis of four representative cases (right), with blue dots as controls of staining. (B) Heat maps showing percentage of IL13Rα2-positive cells in 10 EGFRvIII-positive or -negative (left) and amplified or unamplified EGFR (right) GSC lines. (C) Immunohistochemical stains of IL13Rα2 (brown) and EGFRvIII (red) in a recurrent/residual GBM tissue section. (D) EGFR (upper) and IL13Rα2 (lower) expression segregated by WHO grades (left) and GBM molecular subtypes (right) based on the RNA sequencing data from the TCGA database. The y axis indicates the relative expression levels of each cases. Statistically significant differences were calculated by Kruskal-Wallis test with p < 0.05 being considered statistically significant.