Figure 5.

806BiTE was reluctant in responding to physiologically expressed EGFR

(A) Flow profile showing the expression of EGFR in astrocytes (red), with staining control (blue). (B) 806CAR/BiTE T cells with high (H), medium (M), and low (L) levels of transduction were co-cultured with astrocytes. T cell activation, as measured by CD69 expression, in CD4⁺ and CD8⁺ T cells after 16 h co-culture. (C) Flow based intracellular cytokine (IFNγ, IL2, and TNFα) staining of 806CAR/BiTE T cells co-cultured with astrocytes. CD4- and CD8-positive subgroups of T cells were distinguished by human CD8 staining. (D) T cell activation, as demonstrated by CD69 expression, in CD4⁺ (top) and CD8⁺ (bottom) T cells after 16 h co-culture with target cells and conditioned media of CAR/BiTE T cells. (E) Flow based intracellular cytokine (IFNγ, IL2, and TNFα) staining of UTD T cells co-cultured with astrocytes in conditioned media of CAR/BiTE T cells. Statistically significant differences were determined using log rank test. ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Data are presented as means ± standard deviation.