FIG. 3.
Effect of substrate concentration (Cbz-G-F [A] and Ac-M [B]) on the initial rate (v) of hydrolysis (double-reciprocal plot). The hydrolytic activities were measured in the presence (●) (5 mM) and absence (○) of Ac-M (A) and Cbz-G-G-F (B), at 85°C in 50 mM sodium phosphate buffer (pH 7.5) containing 5% DMF.