TABLE 1.

Substrate specificity of PP for Cbz peptides, Cbz amino acids, peptides, Ac peptides, and Ac amino acids^a

Substrate	Activity (μmol/min/mg of enzyme)	Relative activity (%)
10 mM
↓
Cbz-F	55.9	47
↓
Cbz-G-F	119	100
↓
Cbz-G-G-F	0.571	0.48
↓
Cbz-G	63.0	53
↓
Cbz-R	0.014	0.01
↓
G-F	107b	90
↓
G-G-F	1.21b	1.0
↓
G-G-G-F	0.551b	0.46
↓
Ac-G-F	105a	88
↓
Ac-G-G-F	0.773b	0.65
↓
Ac-G-G-G-F	0.48b	0.40
5 mM
↓
Ac-M	38.1	43
↓
Ac-Y	37.3	29
↓
Ac-F	24.9	27
↓
Ac-A	10.5	15
↓
Ac-W	1.99	2.7
↓
Ac-G	7.40	2.1

^aThe hydrolytic reaction was carried out at 85°C in 50 mM phosphate buffer (pH 7.5) containing 5% DMF. The cleavage site for the substrate is shown by the arrow. 

^bThe hydrolytic activity (release of F) was measured by HPLC.