Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 6;219(8):e20212563. doi: 10.1084/jem.20212563

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Lei et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — HNF4A and HNF4G regulate immune signaling molecules in a location-specific manner. (A) Expression of indicated immune signaling molecules in the ileal and colonic IECs from 3-wk-old mice was determined by qPCR and shown as 2^−dCT. Median. N = 10–12 for each group. (B) RNA-seq analysis of sorted IECs from the ileum and colon of 3-wk-old mice. Volcano plots displaying the fold change and adjusted P value of DE genes enriched in WT (red) and Hnf4a^IEC-KO (blue). DE genes containing HNF4A binding sites within 2 kb of TSS were highlighted (green) and annotated by arrows. Non-DE genes, gray. N = 4 for each group. (C) Percentage of HNF4A direct targets in ileal and colonic DE genes was shown. (D) Principal component analysis of most-varied genes was shown. Each dot represented transcriptome from one sample. Samples of the same genotype and tissue origin were grouped by colored ovals. WT ileum (pink), Hnf4a^IEC-KO ileum (blue), WT colon (orange), and Hnf4a^IEC-KO colon (green). (E) Expression of Btnl1 and Btnl6 along the intestinal tract of young adult mice was determined by qPCR. PS, proximal small intestine; MS, middle small intestine; DS, distal small intestine; CE, cecum; PC, proximal colon; DC, distal colon. Mean with SEM. N = 5–9 for each group. (F) As in E, expression of Hnf4a and Hnf4g along the intestinal tract was determined by qPCR. Mean with SEM. N = 5–9 for each group. (G) Protein levels of HNF4A and HNF4G in the ileal and colonic IECs was determined by Western blot. N = 3 for each group. (H) HNF4G binding to the promoters of indicated genes in the ileal IECs from Hnf4a^IEC-KO mice was determined by ChIP-qPCR and shown as 2^−dCT. Mean with SD. N = 3 for each group. (I) Levels of Btnl1 and Btnl6 in the duodenal IECs of indicated genotypes. Data was adapted from the RNA-seq dataset GSE112946 (Chen et al., 2019). Mean. N = 3 for each group. Each dot represented individual mouse. Data was combined from at least two independent experiments or one representative experiment of two independent experiments and analyzed by Mann-Whitney tests (A, E, F, and H). *, P < 0.0332; **, P < 0.0021; ***, P < 0.0002; ****, P < 0.0001. Source data are available for this figure: SourceData F5.