Table 1.
Antibodies and flow reagents
| Antibodies | Clone/identifier | Source |
|---|---|---|
| Flow cytometry | ||
| CD16/CD32 | 2.4G2 | Tonbo |
| CD326 (EPCAM) | G8.8 | eBioscience |
| CD3 | 17A2 | eBioscience |
| CD4 | RM4-5 | Tonbo |
| CD45.2 | 104 | Tonbo |
| CD8a | 53-6.7 | eBioscience |
| CD8b | YTS156.7.7 | BioLegend |
| CD8b | YTS156.7.7 | BioLegend |
| Ki-67 | SolA15 | eBioscience |
| LAG-3 (CD223) | C8B7W | BioLegend |
| TCR β | H57-957 | eBioscience |
| TCR γ/δ | GL3 | eBioscience |
| TCR Vδ 4 | GL2 | BD Biosciences |
| TCR Vδ 6.3/2 | 8F4H7B7 | BD Biosciences |
| TCR Vγ7 | F2.67 | Dr. Pereira |
| Thy1.2 (CD90.2) | 53-2.1 | BioLegend |
| Streptavidin-BV421 | 405226 | BioLegend |
| Viability dye | 13-0879 | Tonbo |
| Western blot and immunoprecipitation | ||
| Cas9 | 7A9-3A3 | Cell Signaling Technologies |
| HNF4A ChIP grade | EPR16885 | Abcam |
| HNF4A N-terminal | EPR16786 | Abcam |
| HNF4G, polyclonal | HPA005438L | Sigma-Aldrich |
| Rabbit IgG control | Ab171870 | Abcam |
| GAPDH | G9295 | Sigma-Aldrich |