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. 2022 Jun 24;13:930921. doi: 10.3389/fimmu.2022.930921

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Copyright © 2022 Wang, Huang, Zhao, Huang, Tan, Huang, Ma, Xiao, He, Wang, Shen, Lu and Meng

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SAP18-expressing CD8+ T cells co-localize with neutrophil in ccRCC biopsies. (A) Immunofluorescent imaging showing CD8+ T cells in ccRCC biopsies expressing SAP18, and co-localize with neutrophils. CD8+ T cells showing in white, SAP showing in red, neutrophils were labeled with CD66b (green), cell nucleus were counterstained with DAPI. White scale bar indicates 25 μm. (B) Immunofluorescent imaging Neutrophils (CD66b+ cell, green) in ccRCC expressing less ATG7 (red). cell nucleus were counterstained with DAPI. White scale bar indicates 25 μm. (C) Statistical analysis of the frequency of ATG7+ expressing neutrophils against total neutrophils in RCC biopsies. Each dot represented one readout. *: p<0.05. Nonparametric analysis was used.