Table 1.
Detailed UPLC-MS/MS parameters for the determination of probe drugs, their main metabolites, and internal standard.
| Targeted analytes | Precursor → production (m/z) | ESI model (Positive/Negative) | CE(V) | DP(V) | CXP(V) | EP(V) |
|---|---|---|---|---|---|---|
| Omeprazole | 346.1 → 329.0 | Positive | 10 | 30 | 14 | 10 |
| Phenacetin | 180.1 → 110.1 | Positive | 28 | 70 | 13 | 10 |
| Midazolam | 326.1 → 291.2 | Positive | 38 | 120 | 15 | 10 |
| Tolbutamide | 269.1 → 170.1 | Negative | –22 | −55 | −20 | −10 |
| Chlrozoxazone | 168.1 → 132.0 | Negative | −28 | −80 | −15 | −10 |
| Metoprolol | 268.1 → 133.1 | Positive | 34 | 90 | 16 | 10 |
| 5-OH-Omeprazole | 362.1 → 214.2 | Positive | 17 | 45 | 11 | 10 |
| Acetaminophen | 152.1 → 110.1 | Positive | 22.5 | 70 | 13 | 10 |
| OH-Midazolam | 342.1 → 324.1 | Positive | 30 | 50 | 10 | 10 |
| OH-Tolbutamide | 285.1 → 186.1 | Negative | −25 | −60 | −9 | −10 |
| 6-OH-Chlorzoxazone | 183.9 → 120.0 | Negative | −25 | −50 | −12 | −10 |
| α-OH-Metoprolol | 284.0 → 74.1 | Positive | 26 | 90 | 11 | 10 |
| Lamotrigine (IS) | 256.2 → 145.2 | Positive | 45 | 150 | 10 | 10 |
| Glycyrrhetic (IS) | 469.3 → 355.3 | Negative | −59 | −158 | −20 | −10 |
Note: ESI: electrospray ion source; V: voltage; CE: collision energy; DP: declustering potential; CXP: cell exit potential; EP: entrance potential; IS: internal standard.