Table 1.

Detailed UPLC-MS/MS parameters for the determination of probe drugs, their main metabolites, and internal standard.

Targeted analytes	Precursor → production (m/z)	ESI model (Positive/Negative)	CE(V)	DP(V)	CXP(V)	EP(V)
Omeprazole	346.1 → 329.0	Positive	10	30	14	10
Phenacetin	180.1 → 110.1	Positive	28	70	13	10
Midazolam	326.1 → 291.2	Positive	38	120	15	10
Tolbutamide	269.1 → 170.1	Negative	–22	−55	−20	−10
Chlrozoxazone	168.1 → 132.0	Negative	−28	−80	−15	−10
Metoprolol	268.1 → 133.1	Positive	34	90	16	10
5-OH-Omeprazole	362.1 → 214.2	Positive	17	45	11	10
Acetaminophen	152.1 → 110.1	Positive	22.5	70	13	10
OH-Midazolam	342.1 → 324.1	Positive	30	50	10	10
OH-Tolbutamide	285.1 → 186.1	Negative	−25	−60	−9	−10
6-OH-Chlorzoxazone	183.9 → 120.0	Negative	−25	−50	−12	−10
α-OH-Metoprolol	284.0 → 74.1	Positive	26	90	11	10
Lamotrigine (IS)	256.2 → 145.2	Positive	45	150	10	10
Glycyrrhetic (IS)	469.3 → 355.3	Negative	−59	−158	−20	−10

Note: ESI: electrospray ion source; V: voltage; CE: collision energy; DP: declustering potential; CXP: cell exit potential; EP: entrance potential; IS: internal standard.