Table 4.

Pharmacokinetic parameters of probe drugs between control and NASH mice by intravenous administration.

Analytes	Groups	AUC(0-12h), ng·h/mL	AUC(0-infinity), ng·h/mL	Cmax, ng/mL	Tmax, h	t1/2, h	CLz/F, L/h/kg
Omeprazole	Control	335.64 ± 61.51	436.49 ± 128.46	307.00 ± 60.89	0.17 ± 0.11	9.21 ± 8.38	10.91 ± 4.41
	NASH	586.75 ± 178.81**	654.77 ± 150.47	294.57 ± 95.30	0.24 ± 0.09	5.89 ± 5.47	8.02 ± 1.98
5-OH-omeprazole	Control	77.67 ± 63.23	87.05 ± 66.82	113.15 ± 105.36	0.12 ± 0.07	9.36 ± 4.21	116.94 ± 119.32
	NASH	33.53 ± 21.78	43.26 ± 22.92	21.54 ± 20.58	0.28 ± 0.25	9.72 ± 3.13	144.01 ± 67.17
Phenacetin	Control	7017.39 ± 3514.16	7190.82 ± 3361.43	9648.33 ± 4219.11	0.11 ± 0.04	4.76 ± 3.48	0.90 ± 0.59
	NASH	7714.58 ± 3764.24	7857.32 ± 3965.15	10555.71 ± 5268.20	0.15 ± 0.12	3.26 ± 1.85	0.80 ± 0.42
Acetaminophen	Control	81.98 ± 9.56	156.44 ± 65.85	52.93 ± 7.734	0.21 ± 0.07	29.92 ± 11.59	37.72 ± 16.85
	NASH	128.52 ± 42.13**	168.30 ± 38.61	64.46 ± 13.84	0.25 ± 0.13	12.46 ± 12.23	31.02 ± 6.58
Midazolam	Control	3203.76 ± 1650.28	3206.56 ± 1649.65	3594.29 ± 1708.41	0.11 ± 0.04	0.81 ± 0.21	1.97 ± 0.99
	NASH	4749.76 ± 2566.02	4755.99 ± 2567.31	5352.50 ± 2465.10	0.12 ± 0.09	1.21 ± 0.45	1.31 ± 0.61
OH-midazolam	Control	1836.54 ± 490.21	2130.84 ± 824.13	428.86 ± 86.04	1.32 ± 0.59	3.81 ± 1.72	2.55 ± 0.64
	NASH	3561.41 ± 1190.62**	3857.57 ± 1372.45*	694.88 ± 189.24**	1.50 ± 0.38	2.84 ± 1.59	1.45 ± 0.54**
Tolbutamide	Control	25583.23 ± 6932.28	26403.97 ± 7552.17	7807.14 ± 1459.56	0.36 ± 0.44	2.10 ± 0.36	0.20 ± 0.05
	NASH	58276.69 ± 11536.71***	129682.45 ± 63485.35***	9076.25 ± 3163.69	0.16 ± 0.10	12.96 ± 8.82**	0.05 ± 0.03***
OH-Tolbutamide	Control	1329.61 ± 330.20	1414.25 ± 333.57	227.00 ± 35.12	2.92 ± 1.20	2.64 ± 1.89	0.75 ± 0.21
	NASH	2658.69 ± 1322.89*	3218.168 ± 1371.781*	398.50 ± 180.36*	8.00 ± 2.53**	3.99 ± 2.47	0.38 ± 0.20*
Chlorzoxazone	Control	1694.57 ± 1024.63	1700.78 ± 1018.04	3318.00 ± 2218.99	0.14 ± 0.10	1.07 ± 1.83	4.09 ± 2.50
	NASH	1714.34 ± 580.13	1757.71 ± 610.534	3081.43 ± 1836.52	0.14 ± 0.11	1.15 ± 0.68	3.12 ± 0.95
6-OH-chlorzoxazone	Control	123.34 ± 45.67	229.62 ± 195.23	117.40 ± 49.59	0.17 ± 0.10	12.94 ± 18.21	32.43 ± 18.09
	NASH	119.76 ± 33.28	270.50 ± 236.25	58.43 ± 22.65	0.25 ± 0.34	31.13 ± 43.61	26.46 ± 15.86
Metoprolol	Control	3949.38 ± 1727.20	3975.79 ± 1719.27	5518.33 ± 2476.02	0.08 ± 0.00	2.02 ± 1.30	1.52 ± 0.75
	NASH	8342.53 ± 4802.86	8391.23 ± 4795.22	11991.43 ± 8308.01	0.12 ± 0.09	1.83 ± 0.73	0.81 ± 0.50
α-OH-metoprolol	Control	47.31 ± 8.80	76.52 ± 56.13	21.36 ± 3.42	0.21 ± 0.15	5.55 ± 4.57	84.64 ± 34.11
	NASH	64.10 ± 28.85	128.15 ± 191.56	14.95 ± 4.53	1.03 ± 1.33	6.34 ± 8.22	77.34 ± 35.26

Note: AUC_(0-12h): area under the plasma concentration–time curve from 0 to 12 h; AUC_(0-infinity): area under the plasma concentration–time curve from 0 to infinity h; C_max: maximal concentration; t_1/2: eliminate half-life; T_max: time to peak concentration; CLz/F: blood clearance. Data are mean ± SD, n = 8; *p < 0.05, **p < 0.01, ***p < 0.001 versus control group.