Fig. 9.

Effects of exosomes from septic mice on cytokine expression and tissue injury in sepsis. (A) Effects of pretreatment with exosomes from septic mice on the levels of serum cytokines in CLP mice. The levels of IL-1β, IL-6, IL-10 and TNFα were detected with a LiquiChip system as described in the methods section. The data represent three independent experiments (n = 3) and are expressed as the mean with SEM. **: P < 0.01 compared with CLP group. (B) Effects of pretreatment with exosomes from septic mice on the gene expression of cytokines and chemokines in the tissues of CLP mice. The mRNA levels were detected by qRT-PCR (n = 3). The quantitative results for the CLP group and exo-CLP group were divided by those for the sham group, and the ratios were log₂-transformed. (C, D) Effects of pretreatment with exosomes from septic mice on the histopathology of lung, kidney and liver tissues from CLP mice. HE staining (C) was performed with slides of lung, kidney and liver tissues to detect the morphological changes in CLP mice subjected to pretreatment with exosomes from septic mice. Pathological evaluation involved determining the histological score reflecting tissue injury in CLP mice (D). (E) Pretreatment with septic exosomes decreased the Cr levels in the serum of CLP mice.