TABLE 1.
Purification of IDA oxidase
| Purification step | Vol (ml) | Protein (mg) | Total activity (U) | Sp act (U/mg) | Recovery (%) |
|---|---|---|---|---|---|
| Cell extract | 116 | 764 | 118 | 0.16 | 100 |
| Protamine sulfate treatment | 117 | 763 | 115 | 0.15 | 96.9 |
| Ammonium sulfate treatment | 33.2 | 510 | 112 | 0.22 | 94.3 |
| Ultracentrifugation | 32.6 | 344 | 71.7 | 0.21 | 60.6 |
| Phenyl agarose column | 38.0 | 68.5 | 46.7 | 0.68 | 39.5 |
| UnoQ column | 12.0 | 10.1 | 23.4 | 2.33 | 19.8 |
| Hydroxyapatite column | 3.0 | 0.28 | 5.62 | 20.0 | 4.7 |
| UnoQ column | 3.0 | 0.17 | 4.32 | 25.3 | 3.7 |
| Size exclusion column | 1.8 | 0.04 | 1.82 | 47.9 | 1.5 |