REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human CD28/49d (clone L293 and L25)	BD Biosciences	Cat# 347690; RRID: AB_647457
BV421 mouse IgG1, κ isotype (clone ×40)	BD Biosciences	Cat# 562438; RRID: AB_11207319
BV421 anti-human CD127 (clone HIL-7R-M21)	BD Biosciences	Cat# 562436; RRID: AB_11151911
BV510 anti-human CD3 (clone UCHT1)	BD Biosciences	Cat# 563109; RRID: AB_2732053
BV605 anti-human CD8 (clone SK1)	BD Biosciences	Cat# 564116; RRID: AB_2869551
BV786 mouse IgG1, κ isotype (clone ×40)	BD Biosciences	Cat# 563330; RRID: AB_2869484
BV786 anti-human CD38 (clone HIT2)	BD Biosciences	Cat# 563964; RRID: AB_2738515
BV786 anti-human Ki-67 (clone B56)	BD Biosciences	Cat# 563756; RRID: AB_2732007
BV786 anti-human CD3 (clone SK7)	BD Biosciences	Cat# 563800; RRID: AB_2744384
BV786 anti-human IFN-γ (clone 4S.B3)	BD Biosciences	Cat# 563731; RRID: AB_2738391
PE anti-human CD107a (clone H4A3)	BD Bioscience	Cat# 555801; RRID: AB_396135
BB515 anti-human CD45RA (clone HI100)	BD Biosciences	Cat# 564552; RRID: AB_2738841
FITC anti-human CD8 (clone RPA-T8)	BD Biosciences	Cat# 557085; RRID: AB_396580
PerCP-Cy5.5 anti-human CCR7 (clone 150503)	BD Biosciences	Cat# 561144; RRID: AB_10562553
PE anti-human CD95 (clone DX2)	BD Biosciences	Cat# 555674; RRID: AB_396027
PE-CF594 anti-human CD14 (clone MφP9)	BD Biosciences	Cat# 562335; RRID: AB_11153663
PE-CF594 anti-human CD19 (clone HIB19	BD Biosciences	Cat# 562294; RRID: AB_11154408
PE-Cy7 mouse IgG2a, κ isotype (clone MOPC-173)	BD Biosciences	Cat# 560906; RRID: AB_10561847
PE-Cy7 anti-human CD8 (clone RPA-T8)	BD Biosciences	Cat# 557746; RRID: AB_396852
BV421 anti-human Bcl-2 (clone 100)	BioLegend	Cat# 658709; RRID: AB_2563283
PE anti-human Ki-67 (clone Ki-67)	BioLegend	Cat# 350504; RRID: AB_10660752
PE-Cy7 anti-human T-bet (clone 4B10)	BioLegend	Cat# 644824; RRID: AB_2561761
APC-Cy7 anti-human HLA-DR (clone L243)	BioLegend	Cat# 307618; RRID: AB_493586
APC-Cy7 anti-human CD45RA (clone HI100)	BioLegend	Cat# 304128; RRID: AB_10708880
PE-Cy7 anti-human KLRG1 (clone 13F12F2)	Invitrogen	Cat# 25-9488-42; RRID: AB_2573546
Biological samples
Blood samples from individuals with BNT162b2 vaccination	Korea University Guro Hospital, Seoul National University Bundang Hospital	N/A
Chemicals, peptides, and recombinant proteins
CD8 Microbeads, human	Miltenyi Biotec	Cat# 130-045-201
FcR blocking reagent, human	Miltenyi Biotec	Cat# 130-059-901
SARS-CoV-2 S269-277 YLQPRTFLL peptide	Mimotopes	Customized
SARS-CoV-2 S448-456 NYNYLYRLF peptide	Peptron	Customized
SARS-CoV-2 S996-1004 LITGRLQSL peptide	Peptron	Customized
SARS-CoV-2 S1220-1228 FIAGLIAIV peptide	Peptron	Customized
Recombinant human IL-2	Peprotech	Cat# 200-22
Fetal bovine serum	Corning	Cat# 35-015-CV
Lymphocyte separation medium	Corning	Cat# 25-072-CV
Dimethyl sulfoxide	Sigma-Aldrich	Cat# D8418
GolgiPlug (Brefeldin A)	BD Biosciences	Cat# 51-2092KZ
GolgiStop (Monensin)	BD Biosciences	Cat# 51-2301KZ
Critical commercial assays
LIVE/DEAD red fluorescent reactive dye	Invitrogen	Cat# L34972
CellTrace Violet cell proliferation kit	Invitrogen	Cat# C34557
FoxP3/transcription factor staining buffer set	Invitrogen	Cat# 00-5523-00
Software and algorithms
FlowJo software version 10.7	FlowJo LLC	https://www.flowjo.com/
Prism version 9	Graphpad	https://www.graphpad.com/
Other
APC YLQPRTFLL (SARS-CoV-2 S269) HLA-A∗0201 Pentamer	Proimmune	Cat# 4339
APC LITGRLQSL (SARS-CoV-2 S996) HLA-A∗0201 Pentamer	Proimmune	Cat# 4323
APC RLQSLQTYV (SARS-CoV-2 S1000) HLA-A∗0201 Pentamer	Proimmune	Cat# 4358
APC FIAGLIAIV (SARS-CoV-2 S1220) HLA-A∗0201 Pentamer	Proimmune	Cat# 4321
APC NYNYLYRLF (SARS-CoV-2 S448) HLA-A∗2402 Pentamer	Immudex	Customized