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. 2022 Jun 30;16:100347. doi: 10.1016/j.mtbio.2022.100347

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 4 — (A) Result of viability percentage of HDF cells cultured on hydrogel scaffold (HSSO), flat composite scaffold (FCSO) and structural composite scaffold (SCSO) for 7 days. The results are presented as means ± SD, n = 4. Overall HDF cell morphology and orientation when cultured on different composite scaffolds for 7 days. (B) Fluorescent images of HDF cells stained with fluorescein diacetate (FDA) at days 1, 3 and 7 of culture. Scale bar = 200 μm. On the composite scaffolds, most cells elongated and showed a preferred alignment along the direction of the incorporated nanofibre. Quantification of the HDF cell (C) elongation index and (D) the scatter data of cell orientation from at least 30 randomly selected cells. The results are presented as means ± SD, n = 30, (∗p < 0.05 and ∗∗p < 0.01 using one-way ANOVA followed by Tukey's post hoc test).