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. Author manuscript; available in PMC: 2022 Aug 25.
Published in final edited form as: J Am Chem Soc. 2021 Aug 13;143(33):13044–13055. doi: 10.1021/jacs.1c02248

Figure 5.

Figure 5.

Effects of 1, RIBOTAC 4, and LNA-21 on global gene expression in MDA-MB-231 cells. (A) Volcano plot showing transcriptome-wide changes induced by 1 (1 μM, a concentration that does not inhibit pre-miR-21 biogenesis but does inhibit RTKs; n = 3). (B) Volcano plot showing transcriptome-wide changes triggered by RIBOTAC 4 (1 μM; n = 3). (C) Volcano plot showing transcriptome-wide changes caused by LNA-21 (0.1 μM; n = 3). Dotted lines represent adjusted p-value of <0.05. Fold change comparison of 4 vs 1 (D), LNA-21 vs 4(E), and LNA-21 vs 1 (F) suggests that MDA-MB-231 cells treated with RIBOTAC 4 are similar to samples treated with LNA-21 (green, R = 0.80). (43) Both 4 and LNA-21 are dissimilar to samples treated with 1 (blue and red, R = 0.10), as determined by analysis of RNA-seq data.