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. 2022 May 11;135(9):jcs259596. doi: 10.1242/jcs.259596

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Bag6 interacts with MAVS. (A) MAVS co-immunoprecipitates with Bag6. Control KO cells were fractionated as shown in Fig. 2A and the supernatant (S) fraction was subjected to immunoprecipitations with equal amounts of rabbit anti-Bag6 antibody (αBag6) or rabbit IgG antibody (control for non-specific binding). Input and immunoprecipitates were analysed by immunoblotting for the indicated endogenous proteins. SGTA served as positive control for Bag6 binding. (B) SGTA facilitates Bag6–MAVS interaction. Control KO and SGTA KO cells were fractionated as shown in Fig. 2A and the supernatant (S) fractions were subjected to immunoprecipitations with rabbit anti-Bag6 antibody (αBag6) or rabbit control IgG antibody. Inputs and immunoprecipitates were analysed by immunoblotting for the indicated endogenous proteins. SGTA served as positive control for Bag6 binding. Arrow in A and B indicates full-length MAVS. Open circles on MAVS blots indicate signals derived from denatured antibody heavy and light chains. (C) Mean±s.e.m. of MAVS levels that co-immunoprecipitate with Bag6 in control KO and SGTA KO cells for three independent experiments as in B. *P<0.05 (unpaired two-tailed t-test).