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. 2001 Feb;67(2):760–768. doi: 10.1128/AEM.67.2.760-768.2001

TABLE 2.

Specificity of real-time PCR for P. stutzeri DNA

Species, strain, or clonea (genomovar) Sourceb Denitrificationc nirSc Real-time PCRd
Positive controls
 P. stutzeri (1) ATCC 17589 + + +
 P. stutzeri (1) ATCC 27951 + + +
 P. stutzeri (1) ATCC 17593 + + +
 P. stutzeri (1) ATCC 17594 + + +
 P. stutzeri (1) CCUG11256 + −
 P. stutzeri (2) ATCC 17591 + + +
 P. stutzeri (2) ATCC 17587 + + +
 P. stutzeri Zobell (2) ATCC 14405 + + +
 P. stutzeri (2) ATCC 17592 + + +
 P. stutzeri (2) ATCC 17595 + + +
 P. stutzeri (3) ATCC 50227 + + +
 P. stutzeri 19SMN4 (4) DSM 6084 + + −
 P. stutzeri DNSP21 (5) DSM 6082 + + −
 P. stutzeri (7) DSM 50238 + + −
 P. stutzeri JM300 (8) DSM 10701 + + +
 P. stutzeri KC ATCC 55595 + + +
 P. stutzeri A3-5 L. Wu + + +
 P. stutzeri D7-6 L. Wu + + +
 P. stutzeri D9-1 L. Wu + + +
 P. stutzeri E4-2 L. Wu + + +
 P. stutzeri F9-2 L. Wu + + +
Negative controls
 E. coli K-12 DSM 498 − − −
 Paracoccus denitrificans ATCC 17741 + + −
 Azospirillum brazilense DSM 1690 + + −
 Shewanella oneidensis MR-1 ATCC 700550 + + −
 Pseudomonas aeruginosa ATCC 15692 + + −
 Pseudomonas balearica DSM 6083 + + −
 Marine isolate C10-1s L. Wu + + −
 Marine isolate D4-14 L. Wu + + −
 nirS clone A4 G. Braker + + −
 nirS clone B6 G. Braker + + −
 nirS clone A12 G. Braker + + −
 nirS clone B76 G. Braker + + −
a

Marine isolates and nirS clones were obtained from the Washington margin and Puget Sound marine sediments (7). 

b

ATCC, American Type Culture Collection; DSM, Deutsche Sammlung von Mikroorganismen; CCUG, Culture Collection, University of Göteborg; L. Wu, Oak Ridge National Laboratory, Oak Ridge, Tenn.; G. Braker, Michigan State University, East Lansing, Mich. 

c

Reported presence of denitrification and nirS from literature sources, except for results for the characterized genomovars, which were determined in this study by PCR. 

d

+, logarithmic amplification readily detected; −, no amplification detected.