Fig. 2.

Tissue specific expression analysis of apolipophorin and seven candidate Diuraphis noxia effectors (DnE’s). RNA isolated from excised salivary glands or aphid bodies (without heads) was used in reverse transcription semiquantitative PCR with gene specific primers. Preferential salivary gland expression was detected for apolipophorin as well as six DnE’s. Expression of DnC002 and DnSucrase were used as controls for salivary glands and body, respectively. Ribosomal gene L32 was used as an internal control for cDNA input. The numbers on the right indicate PCR cycles at which quantitative differences were observed. The image was compiled from a cropped full-length gel (Additional file 2-Figure S1)