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. 2022 Jul 7;23:493. doi: 10.1186/s12864-022-08712-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Tissue specific expression analysis in D. noxia, through RT-qPCR, of five transcripts predicted to be highly expressed (average Log₂-FC>3) in head tissue through a comparative transcriptomics approach. All five genes were confirmed to have significantly higher expression in heads compared to body tissue (Student’s t-test, P<0.01) when normalised to (A) L27 or (B) L32. APM = Adhesive plaque matrix protein-like, GEMS = Component of Gems protein 1-like, Prisilkin-39 = Prisilkin 39-like, VPS = Vacuolar Protein Sorting-associated Protein TDA6, Skin secretory = Skin Secretory Protein xP2-like. Each bar represents a biological repeat (n=3)